Heteronuclear spin decoupling without optimisation: Methods and recipes
Perunthiruthy Madhu
TIFR Hyderabad, Hyderabad, India

The talk will highlight the current status in heteronuclear spin decoupling in solid-state NMR. We will dwell on the methods and recipes for decoupling under magic-angle spinning frequencies in the range of 5-100 kHz. A key aspect is the ease with which the scheme can be implemented making it nearly parameter independent. The unified strategy that we introduced in this field will be also discussed.

Localized DNP enhancement in biomolecules
Bjoern Corzilius
University of Rostock, Rostock, Germany
View long abstract pdf

Two methods allowing to introduce a large degree of specificity to DNP-enhanced MAS NMR spectra will be presented. The first utilizes the distance dependence of the dipolar hyperfine interaction between the electron spin (source) and nuclear spin (target). In particular, the direct-DNP build-up rate can act as a direct measure of this interaction and can thus yield distance information in biomolecules. The second method employs very efficient indirect DNP (via ¹H), but introduces methyl groups as localized polarization transfer pathways between ¹H and ¹³C. Based on heteronuclear cross-relaxation, SCREAM-DNP can thus probe the local environment around these highly dynamic functions. Examples and applications on proteins, RNA, and ribonucleoproteins (RNPs) will be presented for both effects and future implications discussed.