Code: PH	Time Slot/Poster Number: 125	Session: in vivo MNR Spectroscopy, Poster
Quantification of Alcohol-Induced Brain Metabolites in Mice Using In Vivo 16.4 T MRS
Moonnoh Lee; David Hinton; Prasanna Mishra; John Port; Slobodan Macura; Doo-Sup Choi
Mayo Clinic College of Medicine, Rochester, MN
Abstract
Nuclear magnetic resonance spectroscopy (MRS) has many advantages when investigating in vivo neurotransmitters and metabolite levels in the brain. Here we present the first study that has utilized in vivo 16.4 T MRS to quantify brain metabolites in the nucleus accumbens of mice. In the present study, we found that 3.0 g/kg ethanol significantly reduced myo-inositol, lactate and phosphocreatine when compared to control mice. We also examined the effect of acamprosate, a clinically used medication to treat alcoholism. Interestingly, we found that myo-inositol, lactate and taurine were significantly reduced in response to a 200 mg/kg acamprosate, suggesting that acamprosate treatment mimics acute ethanol administration. Our findings may be useful to understand the molecular basis of alcohol use disorders.

Code: PH	Time Slot/Poster Number: 126	Session: in vivo MNR Spectroscopy, Poster
High-resolution GABA detection with/without J decoupling using 2D multiple-quantum coherence spectroscopy
Xi Chen1, 2; Shaolin Yang1; Laura Rowland2; Yihong Yang1
1National Institute on Drug Abuse, Baltimore, MD; 2Maryland Psychiatric Research Center, Baltimore, MD
Abstract
A 2D modified multiple-quantum coherence sequence is proposed to achieve high-resolution selective GABA detection under inhomogeneous fields. The edited spectra of GABA with and without J splittings can be obtained from the sequence. Sparse sampling in the indirect dimension is utilized to reduce the entire acquisition time. A phantom experiment was performed to demonstrate the feasibility of the proposed method and its potential applications for in vivo studies.

Code: PH	Time Slot/Poster Number: 127	Session: in vivo MNR Spectroscopy, Poster
MSC-SelMQC Method for Simultaneous Mapping of Polyunsaturated Fatty Acids (PUFA), Lactate and Choline in High Fat Tissues
Qiuhong He; Zheng Jing; Gregery Hogan
University of Pittsburgh, Pittsburgh, PA
Abstract
The Selective Multiple Quantum Coherence transfer (Sel-MQC) method is modified for simultaneous mapping of polyunsaturated fatty acids (PUFA), lactate and choline in three unique Molecular Specific Coherence (MSC) transfer pathways with complete lipid and water suppression in a single scan. Choline signal is also detectable in a second spin echo to enhance lipid suppression. The method can be applied to study animal tumor models and human breast cancer or other extracranial cancers in vivo.

Code: PH	Time Slot/Poster Number: 128	Session: in vivo MNR Spectroscopy, Poster
1H MRS Lipids Detection in the Human Calf with Spatially Selective RF Surface Coils
Maria Alfonsetti; Tomas Mazza; Antonello Sotgiu; Marcello Alecci
Università di L'Aquila, L'aquila, Italy
Abstract
We’ll describe the use of figure-of-eight (FO8) transverse coils for in-vivo MRS lipids detection. Calibrations on a 2.35T pre-clinical scanner with phantom showed that the FO8 coil require a less transmit power to obtain the maximum signal value (a factor 5) with significant SNR gains (a factor 3). A circular loop (CL) and a FO8 coil were used on a clinical 1.5T scanner to acquire from calf muscle of volunteers in vivo SPGR images and PRESS spectra from a ROI centrally positioned along the anterior-posterior direction. A number of different peaks corresponding to lipids components were obtained. The peaks obtained with the FO8 coil showed a larger SNR in comparison with the CL coil (a factor 4-6).

Code: PH	Time Slot/Poster Number: 129	Session: in vivo MNR Spectroscopy, Poster
Treatment Response Predictor Using 31P MRS for CHOP and R-CHOP Therapy in Diffuse Large B-Cell Lymphoma
Fernando Arias-Mendoza1; Geoffrey S. Payne2; Kristen Zakian3; Adam J. Schwarz2; Amita Shukla-Dave3; Marion Stubbs4; Nicholas R. Maisey2; Franklyn Howe5; Harish Poptani7; Mitchell R. Smith6; Owen A. O'Connor1; Ruth Pettengell5; Martin O. Leach2; John R. Griffiths4; Jason A. Koutcher3; Jerry D. Glickson7; Arend Heerschap8; Truman R. Brown1
1Columbia University/Radiology, New York, NY; 2Royal Marsden Hospital, Sutton, UK; 3Memorial Sloan-Kettering Cancer Center, New York, NY; 4University of Cambridge, Cambridge, UK; 5St George's Hospital, London, UK; 6Fox Chase Cancer Center, Philadelpha, PA; 7University of Pennsylvania, Philadelphia, PA; 8Radbaud University Medical Centre, Nijmegen, Netherlands
Abstract
In this work we demonstrate that the pretreatment measurement of phosphetanolamine plus phosphocholine normalized to nucleoside triphosphates ([Etn P + Cho P]/NTP) determined noninvasively by 31P MRS predicts treatment outcome in patients with diffuse large B-cell lymphoma treated with standard multi-durg therapy.

Code: PH	Time Slot/Poster Number: 130	Session: in vivo MNR Spectroscopy, Poster
In-cell NMR Spectroscopy with E.coli and S.cerevisiae
Kirsten Robinson; Anne Marie Augustus; Leonard Spicer
Duke University Medical Center, Durham, North Carolina
Abstract
In-cell NMR spectroscopy is a technique that examines proteins at atomic-level resolution within a living cell. Cell-penetrating peptides (CPP’s) have been used to deliver protein into HeLa cells for in-cell NMR. Based on reports that CPP’s can also be imported into E.coli and S.cerevisiae we are exploring this strategy to deliver isotopically labeled protein to enable in-cell studies at controlled protein concentrations more physiologically relevant than overexpression methods. The proteins GB1 and mYPET linked to a CPP are being used to explore protein delivery into E.coli for in-cell NMR. We are also examining the effect of histidine tags on protein detection by NMR using in-cell overexpression of GB1

Code: PH	Time Slot/Poster Number: 132	Session: in vivo MNR Spectroscopy, Poster
Fighting Obesity with Intermolecular Multiple-Quantum Coherences
Rosa Tamara Branca; Warren Warren
Duke University, Durham , NC
Abstract
A water-methylene peak is detected in the iZQC spectrum of BAT. This peak is completely absent in the spectra of WAT and muscle tissue, and can therefore be thought of as a marker for BAT tissue. This approach is insensitive to magnet field inhomogeneities, and allows for an overall in vivo analysis of fat depots displaced in different regions. This method is used to detect the larger BAT depots (>60%) displaced around visceral organs, which are impossible to detect using standard NMR techniques. We also show how this method is able to detect temperature shifts that occurs in BAT upon activation, therefore providing a non-invasive alternative to FDG-PET for detection of BAT activation.