Code: MOE	Time Slot/Poster Number: 3:50-4:40	Session: Tutorial Session
NMR Studies of Membrane Proteins Call For a Comprehensive Approach
Stanley Opella
University of California, San Diego, La Jolla, CA
Abstract
Membrane proteins are challenging systems for NMR spectroscopy because their native phospholipid bilayer environment affects their global dynamics. The development of NMR methods capable of describing the local dynamics and determining the atomic-resolution structures of membrane proteins requires parallel and integrated research in molecular biology and biochemistry, instrumentation, experimental methods, and structure calculations. Comparative studies of membrane proteins in micelle and isotropic bicelle environments by solution NMR provide some insights into local and global dynamics, and are an essential step in the preparation and quality control of the definitive samples of protein-containing phospholipid bilayers for solid-state NMR studies. All steps in the process, starting with the selection of protein targets to the calculation of three-dimensional structures will be discussed.

Code: MOE	Time Slot/Poster Number: 4:40-5:30	Session: Tutorial Session
Spectroscopic Imaging Methods
Andrew Maudsley
University of Miami, Miami, FL
Abstract
While standardized methods for spatially-resolved spectroscopy using single-volume selection methods are widely available on commercial MRI instruments, spectroscopic imaging methods, which combine spectral discrimination and image formation, remain much less widely applied. Spectroscopic imaging methods can include simple two-component spectral discrimination, e.g. fat and water, obtained with high spatial resolution, to multidimensional high-spectral resolution over a volumetric region, and the choice of methods must invariably consider trade-offs in terms of spectral discrimination, spatial resolution, acquisition time, and data quality. Approaches for increasing efficiency of data acquisition and data processing methods for in vivo applications of spectroscopic imaging will be discussed, together with some example applications for clinical studies.

Code: MOE	Time Slot/Poster Number: 5:30-6:20	Session: Tutorial Session
Magnetic Susceptibility in High Resolution NMR and MRI
Thomas M Barbara
OHSU, Portland, OR
Abstract
The bulk susceptibility of an NMR sample and the susceptibility of material in close proximity to it, play an important role in determining frequency shifts and lineshapes. Soon after the development of high resolution methods, NMR was explored as an accurate method for the determination of susceptibility. Other techniques sought to explore compartmentalization or to obtain high resolution spectroscopy from heterogeneous mixtures and suspensions or very small sample volumes of irregular shape. With high field MRI, researchers can now obtain so-called susceptibility weighted images, which provide unique contrast and tissue discrimination. My tutorial will cover the fundamentals of susceptibility in theory and in practice.